Del Mar Photonics

Del Mar Photonics Ti:Sapphire laser Trestles in the UCLA Biophysics Neuroimaging Laboratory

The UCLA Biophysics Neuroimaging Laboratory is committed to developing new microscopy techniques to further the reach of experimental methods in the area of systems neuroscience.

The Lab is collaborating to develop next generation multi-photon microscopy systems, with the aim of imaging millimeter volumes of tissue with non-linear enhanced resolution at millisecond time scales. This may improve methods for analysis of in-vivo cortical circuits and classic microcircuit systems via calcium and potentiometric dyes, provide higher throughput for structural imaging, and enable more detailed modeling of systems of nerve cells. Calcium and potentiometric dyes can be used to monitor network activity with single cell and single spike resolution across hundreds of cells.

Featured in the laboratory is an ultrafast Ti:Sa oscillator kit from Del Mar Photonics. Output power is typically hundreds of milliwatts. KLM Mode locking is started by voice coil and monitor high bandwidth avalanche photodiode combined with a lock-in amplifier.

In this video Adrian Cheng shows his multiphoton imaging system based on Del Mar Photonics Ti:Sapphire laser and describes his experiments on imaging of neural activity in zebra fish.

Del Mar Photonics Ti:Sapphire lasers in combination with a new bread of DPSS and fiber pump lasers bring down the cost of femtosecond laser systems for non-linear imaging and allows more researches to have this powerful research tool in their labs.

The UCLA Biophysics Neuroimaging Laboratory
The UCLA Biophysics Neuroimaging Laboratory is located in Knudsen Hall Room B-171 next to the Physics and Astronomy Building (PAB)

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From DanioRerio:

Highlighting Zebrafish Research at the Society for Neuroscience (SfN) Annual Meeting in San Diego

The Society for Neuroscience (SfN) annual meeting is about to kick off on November 13th. Over 30.000 excited neuroscientists will be browsing the poster sessions, attending symposia, and exchanging ideas. At most conferences that are not zebrafish specific, Danio rerio have to battle for some of the attention. Thankfully, this seems to be getting easier every year. One major indication of zebrafish’ increased popularity in (behavioral) research is the remarkable increase in zebrafish studies showcased at SfN.
This year is my third time attending SfN, and I remember the first time I presented my work very clearly: more than one person was surprised to see behavioral work being done on zebrafish. It’s important to remember that these were all scientists, yet some had still not been familiarized yet with danio in research.

This year I am seeing the same thing I noticed last year: an increase in the number of zebrafish presentations. For the zebrafish community, this is a very exciting development. More of us are using Danio rerio in our research, and the benefits are getting us hooked. The community is taking note, and slowly but surely we are taking a significant spot at major conferences.

Here is a list of presentations (posters and symposia) featuring the zebrafish as a model organism at SfN:
Saturday November 13th 2010
Poster board: 25.3/OOO19
Title: Maze tests and pharmacological manipulation of fear and reward-seeking behavior in zebrafish and other teleosts.
Authors: G. G. Gould, D.J. Echevarria, T.M. Szabo, E.S. Onaivi, T.W. Valenti, and B.W. Brooks.
Date and presentation time (NB: Symposia!): 3:00 PM – 3:15 PM, 13.9, Room 7B.
Title: Expression of a spinocerebellar ataxia type 13 mutant potassium channel subunit in zebrafish impairs execution of the startle response.
Authors: D. M. Papazian, F. A. Issa, C. Mazzochi, J. L. Richardson, J.-Y. Hsieh, A. F. Mock
Poster board: 22.6/NNN28.
Title: University of utah brain awareness 2010.
Authors: D. M. Friend, E. Elias, E. Iacob, C. Mencio, W. Oswald, R. Parker, E. Smith, M. Wright
Time and poster board: 2:00 PM – 3:00 PM, 26.30/OOO73
Title: Initiative to link animal models to human disease (LAMHDI).
Authors: D. Anderson, R. Buccigrossi, M. Ellisman, J. T. Eppig, H. Evans-Kavaldjian, J. S. Grethe, R. Hamel, J. Kemnitz, M. Martone, K. Pohland, N. Preuss, C. L. Smith, M. Sullivan, M. Travers, J. Turner, M. Westerfield, H. Yurt.
Time and poster board: 4-5pm 29.4/A4.
Title: Extracellular nucleotides regulate proliferation of progenitor cells in the adult zebrafish retina during the light:dark cycle.
Authors: M. Ricatti, A. Battista, M. Faillace
Time and poster board: 1-2pm 32.1/D7.
Title: Optogenetic analysis of correlated spontaneous activity in the developing spinal circuit of zebrafish.
Authors: E. Warp, C. Wyart, F. Del Bene, H. Baier, E. Y. Isacoff
Time and poster board: 3-4pm 37.7/F8.
Title: Primary motoneuron axonal pathfinding errors guide secondary motoneuron axonal pathfinding in nicotine-exposed zebrafish.
Authors: K. R. Svoboda, E. Menelaou, L. Paul.
Time and poster board: 2-3pm 37.10/F11.
Title: Expressing alpha7 acetylcholine receptors in a model nicotinic synapse.
Authors: H. Ikeda, J. Urban, F. Ono.
Time and poster board: 3-4pm, 40.23/G32.
Title: Evidence that position 49 plays a role in transmembrane rearrangement during channel opening in P2X4 receptors.
Authors: M. Popova, J. R. Trudell, L. Asatryan, R. L. Alkana, D. L. Davies.
Time and poster board: 1-2pm 42.9/H5.
Title: Large myelinated club endings in embryonic zebrafish contain Cx35.
Authors: C. Yao, S. Nannapaneni, A. E. Pereda.
Time and poster board: 4-5pm 65.16/DD2.
Title: Psychedelic zebrafish: Deconstructing the effects of hallucinogens LSD and MDMA on zebrafish behavior and physiology.
Authors: J. Cachat, J. Dileo, J. Goodspeed, C. Suciu, T. Gilder, K. Wong, A. Stewart, S. Gaikwad, M. Elegante, D. Tien, A. Kalueff.
Time and poster board: 3-4pm 49.7/I36.
Title: Modulators of Tau induced toxicity in a zebrafish model for FTD.
Authors: F. Van Bebber, D. Paquet, A. Hruscha, B. Schmid, S. F. Lichtenthaler, C. Haass.
Sunday November 14th 2010
Time and poster board: 11:00 AM -12:00 PM, 146.8/H30
Title: Disruptions of spatacsin and/or spastizin functions lead to motor neuron axonal outgrowth defects in the Danio rerio.
Authors: E. M. Martin, C. Yanicostas, A. Maouedj, A. Brice, G. Stevanin, N. Soussi-Yanicostas
Time and poster board: 8:00 AM – 9:00 AM, 166.13/BB2
Title: Role of oxytocin/vasopressin system on social behavior and fear response to predator in zebrafish
Authors: D. Braida, A. Finardi, A. Donzelli, V. Capurro, B. Chini, M. Sala
Time and poster board: 4:00 PM – 5:00 PM, 234.16/C38
Title: Characterization of spontaneous electrical activity in developing spinal motor neurons of zebrafish embryos.
Authors: P. V. Plazas, N. C. Spritzer
Time and poster board: 2:00 PM – 3:00 PM, 240.26/F39
Title: Identification and modulation of voltage-gated calcium channels in zebrafish Rohon-Beard neurons.
Authors: Y.-J. Won, F. Ono, S. R. Ikeda
Time and board: 2:00 PM – 3:00 PM, 267.10/HH7.
Title: Effects of ethanol on anxiety-related behaviors in zebrafish (Danio rerio).
Authors: Y. M. Penalosa, R. Blaser
Monday November 15th 2010
Time and poster board: 11:00 AM -12:00 PM, 358.4/V3
Title: Primary neuronal brainstem culture from adult zebrafish: Interactions with an inhibitory chondroitin sulfate proteoglycan-rich environment
Authors: A. Tapanes-Castillo, F. Shabazz, K. Vajn, M. Oudega, J. A. Plunkett
Time and poster board: 8:00 AM – 9:00 AM, 358.5/V4
Title: Neurocan mRNA expression following CNS injury in adult zebrafish (Danio rerio).
Authors: J. A. Plunkett, A. Tapanes-Castillo, F. Shabazz, K. Vajn, M. Oudega
Time and poster board: 9:00 AM -10:00 AM, 358.6/V5.
Title: Molecular and cellular development of scar tissue in the injured spinal cord of adult zebrafish (Danio rerio).
Authors: K. Vajn, A. Tapanes-Castillo, F. Shabazz, J. A. Plunkett, M. Oudega
Time and program (NB: Nanosymposium!): Monday, 2:30 PM – 2:45 PM, 431.7, Room 1B
Title: Touché: A recessive zebrafish mutation which abolishes generator potentials in cutaneous mechanoreceptors.
Authors: S. E. Low, J. Ryan, M. Lachance, L. Saint-Amant
Time and poster board: 3:00 PM – 4:00 PM, 435.19/B9
Title: Methylmercury exposure decreases cell proliferation in the zebrafish (Danio rerio) embryonic neural tube
Authors: E. A. Moussa, S. A. Hassan, L. C. Abbott
Time and poster board: 1:00 PM – 2:00 PM, 439.25/E1
Title: Functional analysis of neuroligin 2 in the developing zebrafish spinal cord
Authors: J. Fierro, JR, C. Davey, A. Tallafuss, P. Washbourne
Time and poster board: 1:00 PM – 2:00 PM, 440.17/E18
Title: Effects of chlorpyrifos exposure on the development of the cholinergic nervous system of the zebrafish and C. elegans
Authors: F. Radoniqi, G. Orona, O. Mac, S. Han, D. Bourgaize, E. A. Fradinger
Time and poster board: 1:00 PM – 2:00 PM, 446.21/G17
Title: Voltage activated currents in zebrafish hair cells
Authors: P. Bravo, B. Escobar, J. Magana, M. Malinao, J. Ngo, O. Sanchez, A. Tucker, M. Valdivia, S. Yaghtin, V. Zeron, B. E. Yazejian
Time and poster board: 4:00 PM – 5:00 PM, 464.12/W8
Title: Behavioral effects of RDX on adult zebrafish
Authors: K. Wong, L. R. Williams, A. Stewart, J. Goodspeed, C. Suciu, J. Cachat, A. V. Kalueff
Time and poster board: 3:00 PM – 4:00 PM, 476.3/MM11
Title: Modulation of the nicotine response
Authors: H. Schneider, S. C. Ekker
Time and poster board: 4:00 PM – 5:00 PM, 478.8/PP18
Title: Integrating convergent evidence across species to find QTL candidate genes using the Ontological Discovery Environment
Authors: V. M. Philip, J. J. Jay, M. A. Langston, E. J. Baker, E. J. Chesler
Tuesday November 16th 2010
Time and poster board: 8:00 AM – 9:00 AM, 573.5/II19
Title: The effects of embryonic etoh exposure on neurochemical and behavioral characteristics of the developing zebrafish
Authors: C. Buske, R. T. Gerlai
Time and poster board: 11:00 AM -12:00 PM, 573.12/JJ6
Title: Ethanol exposure during gastrulation of zebrafish embryogenesis dysregulates microRNAs and their target genes
Authors: D. L. Pappalardo, W.-J. A. Chen, R. C. Miranda
Time and poster board: 8:00 AM – 9:00 AM, 594.17/HHH30
Title: Kisspeptin stimulates electrical activity of extra-hypothalamic gonadotropin releasing hormone (GnRH) neurons from transgenic fish
Authors: Y. Zhao, N. L. Wayne
Time and poster board: 11:00 AM -12:00 PM, 614.16/OOO4
Title: Protein-traps in zebrafish: stressing behavioral genetics
Authors: K. J. Clark, D. Balciunas, N. J. Boczek, A. M. Petzold, E. W. Klee, H. Schneider, K. J. Skuster, M. D. Urban, T. M. Greenwood, S. C. Ekker
Time and poster board: 1:00 PM – 2:00 PM, 636.21/B11
Title: Enhancer screen reveals complex logic for vertebrate dopamine neuron phenotype specification
Authors: J. Bonkowsky, E. Fujimoto, C.-B. Chien
Time and poster board: 2:00 PM – 3:00 PM, 657.18/P4
Title: Anxiolytic and anticonvulsant properties of aqueous Valerian extracts
Authors: L. M. Del Valle-Mojica, B. A. Torres-Hernandez, S. Torres-Rodriguez, C. Rosa-Falero, J. G. Ortiz
Time and poster board: 2:00 PM – 3:00 PM, 706.18/LLL66
Title: Individual differences in zebrafish behavior: Implications for animal models
Authors: C. Toms, D. J. Jouandot, D. J. Echevarria
Time and poster board: 1:00 PM – 2:00 PM, 707.25/MMM27
Title: Assessing attention in the zebrafish
Authors: D. J. Jouandot, C. Toms, D. J. Echevarria
Wednesday November 17th 2010
Time and poster board: Wednesday, Nov 17, 2010, 8:00 AM – 9:00 AM, 745.13/F8
Title: Gaba-a receptor mediated spontaneous electrical activity in anterior dorsal and entopenduncular nuclei in adult zebrafish
Authors: K. Karlsson, Dr., R. A. Vargas